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Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
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Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
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DNASTAR seqbuilder pro version
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
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PRIMER-E permanova+ package
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
Permanova+ Package, supplied by PRIMER-E, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BIOTAGE pyrosequencing assay design software, version 1.0
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
Pyrosequencing Assay Design Software, Version 1.0, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GENETYX CORPORATION genetyx version 10
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
Genetyx Version 10, supplied by GENETYX CORPORATION, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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EpiCypher cut run library prep kit
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
Cut Run Library Prep Kit, supplied by EpiCypher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RStudio software r studio version 1.0.136
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
Software R Studio Version 1.0.136, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad pcr array data analysis software
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
Pcr Array Data Analysis Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GENETYX CORPORATION primer 6 software version 10
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
Primer 6 Software Version 10, supplied by GENETYX CORPORATION, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Idaho Technology Inc lightcycler probe design software, version 1.0
Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in <t>Geneious</t> Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.
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Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in Geneious Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.

Journal: Viruses

Article Title: First Molecular Evidence of the Presence of Avian Astroviruses in Turkey Flocks of Ecuador Through the Standardization of RT-qPCR Assays Based on SYBR Green

doi: 10.3390/v18030308

Figure Lengend Snippet: Phylogenetic analysis of TAstV isolates based on partial ORF1 gene fragment sequences generated in the present study, together with reference sequences retrieved from GenBank and organized by their country of origin. Multiple sequence alignment was performed using the Clustal Omega package implemented in Geneious Prime v2.0.10 ( https://www.geneious.com ). The phylogenetic tree was inferred with the MEGA 11 software package, applying the maximum likelihood method under the Tamura–Nei substitution model. Bootstrap support values (1000 replicates) are displayed along the branches. The scale bar indicates the number of substitutions per nucleotide site. Sequences obtained in this work are highlighted with circles, ANV sequences are depicted in red, TAstV-1 sequences in green, and TAstV-2 sequences in blue.

Article Snippet: Electropherograms were processed using the Geneious Prime software package, version 10.2.3 ( https://www.geneious.com , accessed on 16 January 2026) (Biomatters Ltd., Auckland, New Zealand).

Techniques: Generated, Sequencing, Software